LOCUS       sequence-97887-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP from jellyfish Aquorea sp. under T5 promoter with lac operators;
            ampicillin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p007ampGFP
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..71
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    112..131
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(137..143,144..160,161..166,167..181))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    206..222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(678..695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(678..694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(793..810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtctaattca acaagaattg ggacaactct agtgaaaagt tcttctcctt tactcatttt
       61 ttacctcctt aaaagttaaa caaaattatt tctagaggga aaccgttgtg gaattgtgag
      121 cgctcacaat tccacatatt ataattgtta tccgctcaca aagcaaataa atttttcatg
      181 atttcactgt gcatgaagct cgtaattgtt atccgctcac aattaagggc gacacaaaat
      241 ttattctaaa tgataataaa tactgataac atcttatagt ttgtattata ttttgtatta
      301 tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt
      361 tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa
      421 tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa
      481 gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac
      541 aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc
      601 gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca
      661 gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc
      721 aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc
      781 gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      961 gtttttccat agg
//
