LOCUS       sequence-97888-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP from coral Corynactis sp. under T5 promoter with lac operators; 
            kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p005kanRFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     69..88
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aataggggtc agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat
       61 acctgaatat ggctcataac accccttgtt tgcctggcgg cagtagcgcg gtggtcccac
      121 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc
      181 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      241 tgggcctttc gcccgggcta attagggggt gtcgcccttg gggttacgtt tctttaacgt
      301 cgacggtgaa gtgctttgcg accgcatgct cttggatctg aaagccgtcg gcaacctctt
      361 ttttaacaag cacgtgttcg ataatgtgat tcggcggaat cttaactgcc ttgtgggctt
      421 tgtagctggt ggtgaactga catttaagac ggtggccacc ttccagcaac agaaacatgg
      481 tcacatcgcc tttcaggata ccgtcgcacg ccgtaatcag ttcgctgctc ggctcccagt
      541 tcagcgtttt cttttgcatg accggaccgt ctgccgggaa attgacgcca tggaaggtgc
      601 tattgtgaat gaagcagttc ttatccagac taatgtgcgc gctcgccgta ccacaaccgc
      661 catcctcaaa catcagggtg cgttcccaag agtaaccttc cggcaggctc tgtttaaagt
      721 agtccggcat gtcctctgga taatcgcaaa aacaacggtt accgtaagta aaaacagagc
      781 tcaggatatc gaacgcaaac ggcagcgggc ca
//
