LOCUS       sequence-97890-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP from coral Corynactis sp. under T5 promoter with lac operators; 
            kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p005kanRFP
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    204..220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(245..259,260..265,266..282,283..289)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    295..314
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             355..363
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
       61 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
      121 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atgcatttag
      181 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
      241 gaaatcatga aaaatttatt tgctttgtga gcggataaca attataatat gtggaattgt
      301 gagcgctcac aattccacaa cggtttccct ctagaaataa ttttgtttaa cttttaagga
      361 ggtaaaaaat gtccctgtcg aaacaagtac tgccacgtga tgttcgcatg cgctttcata
      421 tggatggctg cgttaatggt catcagttca tcattgaggg tgaaggtaca ggcaaaccgt
      481 atgagggcaa gaagattctg aaactgcgcg tgacgaaagg tggcccgctg ccgtttgcgt
      541 tcgatatcct gagctctgtt tttacttacg gtaaccgttg tttttgcgat tatccagagg
      601 acatgccgga ctactttaaa cagagcctgc cggaaggtta ctcttgggaa cgcaccctga
      661 tgtttgagga tggcggttgt ggtacggcga gcgcgcacat tagtctggat aagaactgct
      721 tcattcacaa tagcaccttc catggcgtca atttcccggc agacggtccg gtcatgcaaa
      781 agaaaacgct gaactgggag ccgagcagcg aactgattac ggcgtgcgac ggtatcctga
      841 aaggcgatgt gaccatgttt ctgttgctgg aaggtggcca ccgtcttaaa tgtcagttca
      901 ccaccagcta caa
//
