LOCUS       sequence-97891-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP from jellyfish Aquorea sp. under T5 promoter with lac operators;
            kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p006kanGFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(215..234)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        301..392
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatgaacta tacaaataac cccaagggcg acacccccta attagcccgg gcgaaaggcc
       61 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
      121 gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
      181 aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag ccatattcag
      241 gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca ctgaccccta
      301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      361 aaatgcttca ataatattga aaaaggaaga atatgagcca tattcaacgg gaaacgtcga
      421 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      481 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
      541 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      601 taaactggct gacggaattt atgccacttc cgaccatcaa gcattttatc cgtactcctg
      661 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcgttccag gtattagaag
      721 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      781 actcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc ctcgctcag
//
