LOCUS       sequence-97893-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP from jellyfish Aquorea sp. under T5 promoter with lac operators;
            kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p006kanGFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..69
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    110..129
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(135..141,142..158,159..164,165..179))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    143..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    204..220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(677..694)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(677..693)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctaattcaac aagaattggg acaactctag tgaaaagttc ttctccttta ctcatttttt
       61 acctccttaa aagttaaaca aaattatttc tagagggaaa ccgttgtgga attgtgagcg
      121 ctcacaattc cacatattat aattgttatc cgctcacaaa gcaaataaat ttttcatgat
      181 ttcactgtgc atgaagctcg taattgttat ccgctcacaa ttaagggcga cacaaaattt
      241 attctaaatg cataataaat actgataaca tcttatagtt tgtattatat tttgtattat
      301 cgttgacatg tataattttg atatcaaaaa ctgattttcc ctttattatt ttcgagattt
      361 attttcttaa ttctctttaa caaactagaa atattgtata tacaaaaaat cataaataat
      421 agatgaatag tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag
      481 tgcgttgctt ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca
      541 ggcgccctta aatattctga caaatgctct ttccctaaac tccccccata aaaaaacccg
      601 ccgaagcggg tttttacgtt atttgcggat taacgattac tcgttatcag aaccgcccag
      661 ggggcccgag cttaagactg gccgtcgttt tacaacacag aaagagtttg tagaaacgca
      721 aaaaggccat ccgtcagggg ccttctgctt agtttgatgc ctggcagttc cctactctcg
      781 ccttccgctt cctcgctc
//
