LOCUS       sequence-97914-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nuclear mTagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-N-P-M-nucBFP-EGFP-L
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..172
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(308..328)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(332..352)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(356..376)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
       61 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      121 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      181 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcggccgta cgggaggggt
      241 gttagttttt ttcatggact tggatcgttg aaaggacgtt aattaattat ctagatccgg
      301 tggatcctac ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct
      361 ttctcttctt ttttggatca gctcgagatc tgagtccgga attaagcttg tgccccagtt
      421 tgctagggag gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg taggtctcgt
      481 tgttggcctc cttgattctt tccagtctgt agtccacata gtagacgcca ggcatcttga
      541 ggttcttagc gggtttcttg gatctatatg tggtcttgat gtttgc
//
