LOCUS       sequence-97916-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Yoon JY, Lee SJ, Kim DJ, Lee BJ, Yang JK, Suh SW
  TITLE     Crystal structure of JHP933 from Helicobacter pylori J99 shows 
            two-domain architecture with a DUF1814 family nucleotidyltransferase
            domain and a helical bundle domain.
  JOURNAL   Proteins. 2014 Mar 27. doi: 10.1002/prot.24572.
  PUBMED    24677396
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2014 Mar 27. doi: 10.1002/prot.24572."
COMMENT     Sequence Label: pET28b(+)-JHP933
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatt atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggatagc aaaaaacccc attatagagt
      121 gagtttaagc gaacaagctc ttaatcatga aaaactgatg agagccattg ttaaaaatct
      181 agccgacacc cctatggtat taaaaggcga aacagctttg tatctaggct atggcttaaa
      241 tcgtttttca gaagatttag actttgattg tcataaaaaa atcaatctct taggcagagt
      301 aaagagcgct attcctaatg gcattatctt aaatgatatt catattaaaa aagacactga
      361 tagtgtgggg cgttatatgg tgcgttatgc taccaaagac aataaagaag aacaaacttt
      421 aaaattagaa atatcttata gagacgcacc aaaagagagc gaagtcaatg tgattgaggg
      481 aatgcgaatt gctaaaatag agcgtattat tgataacaaa ctctgtgctt gctttgatgg
      541 ggagcataca
//
