LOCUS       sequence-97921-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             262..282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             286..306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
       61 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
      121 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
      181 gacctcccta gcaaactggg gcacaagctt aattccggac tcagatctcg agctgatcca
      241 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga
      301 aaggtaggat ccaccggatc tagataatta attaacgtcc tttcaacgat ccaagtccat
      361 gaaaaaaact aacacccctc ccgtacggcc gccaccatgg acgtggtgaa tcagctggtg
      421 gctgggggtc agttccgggt ggtcaaggag ccccttggct tcgtgaaggt gctgcagtgg
      481 gtctttgcca tcttcgcctt tgctacgtgc ggcagctaca ccggagagct tcggctgagc
      541 gtggagtgtg ccaacaagac ggagagtgcc ctcaacatcg aagtcgaatt tgagtaccca
      601 ttcaggctgc accaagtgta ctttgatgca ccctcctgcg ttaaaggggg cactaccaag
      661 atcttcctag ttggtgacta ctcctcctcg ggtgaattct ttgtcaccgt ggctgtgttt
      721 gccttcctct actccatggg ggccctggcc acctac
//
