LOCUS       sequence-97922-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts GFP immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-GFP
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggtaccga gctcggatcc
       61 actagtaacg gccgccagtg tgctggaatt cgcccttttc ctgcaggctc tgaagacctg
      121 cctcttcaag gcagccctga ttcttctacc agtcccaaac aaaagctctc aagtcctgag
      181 gctgacaagg gccctgagga ggaggagaac aaggtccttg ccaggaagca gaagatgcgg
      241 actgtgttct ctcaggccca gctgtgtgca ctcaaggaca ggtttcagaa gcagaagtac
      301 ctcagcctcc agcagatgca agaactctcc tccattctga acctgagcta taagcaggtg
      361 gggtccttag aagttgctgt aatgtaacac aatgagactg acttgctaat gaatcgaact
      421 aacgtctgga cgtctctcta actatcctat cctcctatcc tatgcgcctg ttaaaatcat
      481 aattatgtga ttgattgatt gattgattat gtataagtac actatagctg tcttcagata
      541 caccagaaga gggcatcaga tctcattacg gatggttgtg agccaccatg tggttgctgg
      601 gatttgaact caggaccttc ggaagagcag tcagtgctct taaccgctga gccatctctc
      661 cagccccaat tatgtgattt atatatttcc cagtttgttt cattggttac tttatcttct
      721 acttcacctc ccacacccat ccccaaatca gggtctcact gtagccctga ctggtctctg
      781 tagaccaggc tgggagaggt tcacctgcct ctgtctcctg ggtgctggaa ataaaggtgt
      841 gcctggcttt tttcctttct tttctttttt aagattgttt atttttaaat tcctttagtt
      901 catt
//
