LOCUS       sequence-97928-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts mCherry immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..173)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccattggc
      121 ccgggattct cttcgacatc ccctgcttgt ttcaacaggg agaagttagt ggcgctagct
      181 atttcacctg gtggagtcac agagtagttc aggaataatt ccaaggcttg tggggtgcta
      241 aaatgcgcat ggctttccct agtggcttcc aaattcacct ccaaatcact ggcagagaag
      301 ttttgctgca actgtacgta aggctgcaga aagtcctccc cgaagttatg gagcggagca
      361 gcattccaag gctggccgtt ccaggactga gcggtccagg cctggctgct ccaagttggg
      421 ttggtccagg tctggttgtt ccaagttggg ttggtccagg tctggctgct ccaagttggg
      481 ttggtccaag tctggctgcc ccacatggaa aggcttccag atgcgttcac cagatagccc
      541 tggggatagc tgcaatggat gctgggatac tccactggtg ctgagccctt ctgaaagagg
      601 tgattaatgc aatgagtgaa aaggggactc taaagatgta aactgcagaa atactgaaaa
      661 gattagcatg gccgcacagg ccgttaaccc cagcactcct gagactaggc aggcagatct
      721 ctgactgcca gggctacata gagagagcct gcctcaaaga accaaagatc acaagaaaga
      781 gtgcgtggag gaagagggat gggaaaggga caggactaat ggacataga
//
