LOCUS       sequence-97930-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nuclear EGFP and Tomato in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Yoo AS, Staahl BT, Chen L, Crabtree GR
  TITLE     MicroRNA-mediated switching of chromatin-remodelling complexes in 
            neural development.
  JOURNAL   Nature. 2009 Jul 30;460(7255):642-6. doi: 10.1038/nature08139. Epub 
            2009 Jun 28.
  PUBMED    19561591
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08139"; journalName: "Nature"; date: "2009-07-30- 
            30"; volume: "460"; issue: "7255"; pages: "642-6"
COMMENT     Sequence Label: pNSEN-d2
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     143..166
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             259..279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             283..303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             307..327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    458..579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(495..514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     549..568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(723..744)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
       61 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
      121 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
      241 ctgtacaagg ccgtggatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
      301 gtagatccaa aaaagaagag aaaggtacac ggctaagcgg ccgcgactct agatcataat
      361 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      421 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
      601 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      661 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      721 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
//
