LOCUS       sequence-97931-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nuclear EGFP and Tomato in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Yoo AS, Staahl BT, Chen L, Crabtree GR
  TITLE     MicroRNA-mediated switching of chromatin-remodelling complexes in 
            neural development.
  JOURNAL   Nature. 2009 Jul 30;460(7255):642-6. doi: 10.1038/nature08139. Epub 
            2009 Jun 28.
  PUBMED    19561591
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08139"; journalName: "Nature"; date: "2009-07-30- 
            30"; volume: "460"; issue: "7255"; pages: "642-6"
COMMENT     Sequence Label: pNSEN-d2
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(289..309)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(313..333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(337..357)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(421..617)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(429..639)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      435..570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gacacgtgta cctttctctt
      301 cttttttgga tctacctttc tcttcttttt tggatctacc tttctcttct tttttggatc
      361 cacgtgcacc atggtggcga ccggtcgtgg cggccgacct gcaggcatgc aagctagctt
      421 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
      481 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
      541 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
      601 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttc
//
