LOCUS       sequence-97944-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: MSCV-beta-catenin-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..133
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattggcca ccatggctac
       61 tcaagctgac ctgatggagt tggacatggc catggagccg gacagaaaag ctgctgtcag
      121 ccactggcag cagcagtctt acttggatgc tggaatccat gctggtgcca ccgccacagc
      181 tcctgccctg agtggcaagg gcaaccctga ggaagaagat gttgacacct cccaagtcct
      241 ttatgaatgg gagcaaggct tttcccagtc cttcacgcaa gagcaagtag ctgatattga
      301 cgggcagtat gcaatgacta gggctcagag ggtccgagct gccatgttcc ctgagacgct
      361 agatgagggc atgcagatcc catccacgca gtttgacgct gctcatccca ctaatgtcca
      421 gcgcttggct gaaccatcac agatgttgaa acatgcagtt gtcaatttga ttaactatca
      481 ggatgacgcg gaacttgcca cacgtgcaat tcctgagctg acaaaactgc taaacgatga
      541 ggaccaggtg gtagttaata aagctgctgt tatggtccat cagctttcca aaaaggaagc
      601 ttccagacat gccatcatgc gctcccctca gatggtgtct gccattgtac gcaccatgca
      661 gaatacaaat gatgtagaga cagctcgttg tactgctggg actctgcaca acctttctca
      721 ccaccgcgag ggcttgctgg ccatctttaa gtctggtggc atcccagcgc tggtgaaaat
      781 gcttgggtca ccagtggatt ctgtactgtt ctacgccatc acgacactgc ataatctcct
      841 gctccatcag gaaggagcta aaatggcagt gcgcctagct ggtggactgc agaaaatggt
      901 tgctttgctc aacaaaa
//
