LOCUS       sequence-97948-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Sheen JH, Zoncu R, Kim D, Sabatini DM
  TITLE     Defective Regulation of Autophagy upon Leucine Deprivation Reveals a
            Targetable Liability of Human Melanoma Cells In Vitro and In Vivo.
  JOURNAL   Cancer Cell. 2011 May 17. 19(5):613-28.
  PUBMED    21575862
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2011 May 17. 19(5):613-28."
COMMENT     Sequence Label: pQCXI Puro DsRed-LC3-GFP
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(174..193)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     670..690
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
      181 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
      241 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
      301 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      361 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
      421 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      481 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      541 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      601 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      661 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgt
//
