LOCUS       sequence-97950-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Sheen JH, Zoncu R, Kim D, Sabatini DM
  TITLE     Defective Regulation of Autophagy upon Leucine Deprivation Reveals a
            Targetable Liability of Human Melanoma Cells In Vitro and In Vivo.
  JOURNAL   Cancer Cell. 2011 May 17. 19(5):613-28.
  PUBMED    21575862
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2011 May 17. 19(5):613-28."
COMMENT     Sequence Label: pQCXI Puro DsRed-LC3-GFP
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GS"
     primer_bind     511..530
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacggacc tgcaggaatt gatccgcggc cgctgagcca ccatggacaa
      121 caccgaggac gtcatcaagg agttcatgca gttcaaggtg cgcatggagg gctccgtgaa
      181 cggccactac ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac
      241 cgccaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      301 ccagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactacat
      361 gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc atgaacttcg aggacggcgg
      421 cgtggtggag gtgcagcagg actcctccct gcaggacggc accttcatct acaaggtgaa
      481 gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga ctgccggctg
      541 ggagccctcc accgagaagc tgtaccccca ggacggcgtg ctgaagggcg agatctccca
      601 cgccctgaag ctgaaggacg gcggccacta cacctgcgac ttcaagaccg tgtacaaggc
      661 caagaagccc gtgcagctgc ccggcaacca ctacgtggac tccaagctgg acatcaccaa
      721 ccacaacgag gactacaccg tggtggagca gtacgagcac gccgaggccc gccactccgg
      781 ctccca
//
