LOCUS       sequence-97969-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet inactive enhancer, a 
            minimal promoter and a luc2 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23 C2_14
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(592..616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        690..721
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcccgtcga cgaattctgc agatatcaca
       61 agtttgtaca aaaaagcagg ctccgcggcg cccacccttc accttctgca atgtttaatc
      121 tgcctttaat tccatccagt gtatttgtct tctcataatt tgtcatttct tctctagaac
      181 tttgatttgg gtattttaaa aatacatccc atatttctac tttaacttct tgaacacatg
      241 aaatgcaatt ttgatagcat ctaaacataa ttctaaatgt aacagatata acgctaacat
      301 cggtgtcagt tctgggacag ttacgattga ttactctctc attatgggtc atatttttct
      361 gcctcttcgc atgcctggaa atctttggtt ggctgccaga cattgtgact ttatcttgct
      421 gggtgctgga tatttttata ttcctataaa tcttcttggg ctttgctttt tgaaaacagt
      481 tggatcttct cgtgtcttgc ttttaagatt tgttaggtgg gtgtggagca gtgctctgac
      541 agaagctaat gactccctac cactgagctg agaccaaggg tgggcgcgcc gacccagctt
      601 tcttgtacaa agtggtgata aacccgctga tcagcctcga ctgtgctcga ggatatcaag
      661 atctggcctc ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca
      721 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaa
//
