LOCUS       sequence-97977-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. 
            Contains KrasG12D HDR donor. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre
            -shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1003..1023)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catggccccg gtttcagagt caagattgcg gatatagttc ataacgatgt tcacatttgt
       61 ccaacctcca gcttgcataa tctctggtat ggagactcct gcccgcgcca tgtctcgtgc
      121 ggcccccacc ctagcagaat gacctgacca ggcgagatac ctctggccac tgtcatcctt
      181 ggctccgtat atgagtcggt gggttgcctc aaagatccct tcaagggccc gtgtactcaa
      241 ttggctggtg gcagagggcg cggcgactcc gttttttctc actcgacaga acaagtaatt
      301 attagggtcg tcagccacgc cggacactga gatccatctt tccaccagct tcgttacgcc
      361 cagggacaag gccttttcaa cgccagcggt gctgaccaat gtctttgtgc gtccaatatg
      421 aataagcatc cggccgccgt ccgttcgact gatgtctttc actctgatcc gggcgatctc
      481 agctatgcgc agaagtgtgt tatatgcaat tcccagaaat gcgaggttcc tgatgtcctg
      541 gcaccgatcg ctgttctcca tcagggatct gacctggtcg aaatcagtcc gctcaaaagc
      601 gagagcctgc ttagccctct cacccgcatc tacgttttcc tttctaattc tcctcataac
      661 aagggagact gcgtttgaat cgctaggccg ggggagcccg ctgcgtcgat gcagcatatt
      721 cagttggccc aagtgctgct ggatagtctt gacagccagc cctcttgcct ggagatacag
      781 caggtaatcg cgcacgtcct ccggctcggc ggggaaccat ttgcgattgt tcagcttaca
      841 ccaggctgcc caggatcggc acacgctaag cagcattttc caggtgtgtt cagaaaacgc
      901 ctgtctatcc ctaaacatat ccatgagatt tttcctgacc tcatcgctag tggcatctac
      961 tggcagcgca gggaggttct ggtgaacagt caggagattg gacaccttcc tcttcttctt
     1021 gggtggcccg ggattctctt cgacatcccc tgcttgtttc aacagggaga a
//
