LOCUS       sequence-97980-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. 
            Contains KrasG12D HDR donor. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre
            -shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(765..791)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggacatcaca tataactgaa ataactttca atataatttt ctaataaata taaaaatgat
       61 atctttttca aagcggctgg ctgccgtcct ttacaagcgc acgcagactg tagagcagcg
      121 ttacctcgat ggttggatca tactcatcca caaagtgatt ctgaattagc tgtatcgtca
      181 aagcgctctt gcccacgccg tcggcgccca cgaccacaag tttatactca gtcattttca
      241 gcaggcctta caataaaaat aataaaatac aactaaatta gaacatgtct cacacaagat
      301 tatcaaaaac tttatcaata ttttaacact cacctttgtg tgtaaaactc taagatattc
      361 cgaattcagt gactacagat gtacagagta acctgtaagt cacttacaac tttctcatca
      421 acacttaata aaaatacatg gaaaccagta ctttcatctt ctatcattta gcttgttgat
      481 ctaaacagcc agattactgt tttgtagcag ctaatggctc tcaaaggaat gtatcatgac
      541 ttcactcagt acaaatattc tgcatagtac gctataccct gtggacacac ccgcatgagc
      601 ttgtcgacag ctatcccaac acctaccctt gcggtattct ttgttgactg gatattaaaa
      661 gttagaagtt aggtagccta agaacatctg tgttggagcc aaagcttaca caaaaaacca
      721 acacacagat ctaatgaaaa taaagatctt ttattagcgc tttaagcgta atctggaaca
      781 tcgtatgggt agtcaccatc ttcgagcagt ctcaccatgg ccccggtttc agagtcaaga
      841 ttgcggatat agttcataac gatgttcaca tttgtccaac ctccagcttg cataatctct
      901 ggtatggaga ctcctgcccg cgccatgtct cgtgcggccc ccaccctagc agaatgacct
      961 gaccaggcga gatacctctg gccactgtca tccttggctc cgtatatgag tcggtgggtt
     1021 gcctcaaaga tcccttcaag ggcccgtgta ctcaattggc tggtggcaga gggcgcggcg
     1081 actccgtttt
//
