LOCUS       sequence-97982-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. 
            Contains KrasG12D HDR donor. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre
            -shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..198
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             199..219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa
       61 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat
      121 gaacaagcta gcggaagcgg agccactaac ttctccctgt tgaaacaagc aggggatgtc
      181 gaagagaatc ccgggccacc caagaagaag aggaaggtgt ccaatctcct gactgttcac
      241 cagaacctcc ctgcgctgcc agtagatgcc actagcgatg aggtcaggaa aaatctcatg
      301 gatatgttta gggatagaca ggcgttttct gaacacacct ggaaaatgct gcttagcgtg
      361 tgccgatcct gggcagcctg gtgtaagctg aacaatcgca aatggttccc cgccgagccg
      421 gaggacgtgc gcgattacct gctgtatctc caggcaagag ggctggctgt caagactatc
      481 cagcagcact tgggccaact gaatatgctg catcgacgca gcgggctccc ccggcctagc
      541 gattcaaacg cagtctccct tgttatgagg agaattagaa aggaaaacgt agatgcgggt
      601 gagagggcta agcaggctct cgcttttgag cggactgatt tcgaccaggt cagatccctg
      661 atggagaaca gcgatcggtg ccaggacatc aggaacctcg catttctggg aattgcatat
      721 aacacacttc tgcgcatagc tgagatcgcc cggatcagag tgaaagacat cagtcgaacg
      781 gacggcggcc ggatgcttat tcatattgga cgcacaaaga cattggtcag caccgctggc
      841 gttgaaaagg ccttgtccct gggcgtaacg aagctggtgg aaagatggat ctcagtgtcc
      901 ggcgtggctg acgaccctaa taattacttg ttctgtcgag tgagaaaa
//
