LOCUS       sequence-97984-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of WDR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, 
            Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D
  TITLE     A role for WDR5 in integrating threonine 11 phosphorylation to 
            lysine 4 methylation on histone H3 during androgen signaling and in 
            prostate cancer.
  JOURNAL   Mol Cell. 2014 May 22;54(4):613-25. doi: 
            10.1016/j.molcel.2014.03.043. Epub 2014 May 1.
  PUBMED    24793694
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.043"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "613-25"
COMMENT     Sequence Label: pSuper.Retro.Puro-WDR5 shRNA 2
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(93..307)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(153..172)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        318..817
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(353..376)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     750..769
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(842..861)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gatctctcga ggtcgacggt atcgataagc ttaaaaagaa agagattgta cagaaatctc
       61 ttgaatttct gtacaatctc tttcggggat ctgtggtctc atacagaact tataagattc
      121 ccaaatccaa agacatttca cgtttatggt gatttcccag aacacatagc gacatgcaaa
      181 tattgcaggg cgccactccc ctgtccctca cagccatctt cctgccaggg cgcacgcgcg
      241 ctgggtgttc ccgcctagtg acactgggcc cgcgattcct tggagcgggt tgatgacgtc
      301 agcgttcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      361 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      421 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      481 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      541 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
      601 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
      661 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
      721 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
      781 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gctagcttac
      841 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
      901 acgcaccctc gccgccgcgt tcg
//
