LOCUS       sequence-97986-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of WDR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, 
            Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D
  TITLE     A role for WDR5 in integrating threonine 11 phosphorylation to 
            lysine 4 methylation on histone H3 during androgen signaling and in 
            prostate cancer.
  JOURNAL   Mol Cell. 2014 May 22;54(4):613-25. doi: 
            10.1016/j.molcel.2014.03.043. Epub 2014 May 1.
  PUBMED    24793694
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.043"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "613-25"
COMMENT     Sequence Label: pSuper.Retro.Puro-WDR5 shRNA 2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(146..562)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(162..178)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(194..216)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gtctttggat ttgggaatct tataagttct gtatgagacc acagatcccc gaaagagatt
       61 gtacagaaat tcaagagatt tctgtacaat ctctttcttt ttaagcttat cgataccgtc
      121 gacctcgaga gatcgatcta attccggcgc ctagagaagg agtgagggct ggataaaggg
      181 aggatcgagg cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa
      241 gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca
      301 aggcttccca ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa
      361 agaccttgat cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc
      421 cgacgttaaa ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat
      481 ctaccgactg gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta
      541 agggagtggt aacagtctgg ccctaatttt cagacaaata cagaaacaca gtcagacaga
      601 gacaacacag aacgatgctg cagcgctgca gcagacaaga cgcgcggctt cggttccaaa
      661 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
      721 ccacatatcc tgacggggtc ggattccaca tcgactccct tcctcaggtc gggccacaaa
      781 aacggccccc aaagtccctg ggacgtctcc cagggttgcg gccgggtgtt cagaactcgt
      841 cagttccacc acgggtccgc cagatacaga gctagttagc taactagtac agacgcaggc
      901 gcaaacatta gatgccggca caaacacgca caa
//
