LOCUS       sequence-97987-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. 
            Contains KrasG12D HDR donor. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre
            -shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..1033
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
       61 cgcaacgggt ttgccgccag aacacaggcg tacggccacc atgacttcga aagtttatga
      121 tccagaacaa aggaaacgga tgataactgg tccgcagtgg tgggccagat gtaaacaaat
      181 gaatgttctt gattcattta ttaattatta tgattcagaa aaacatgcag aaaatgctgt
      241 tattttttta catggtaacg cggcctcttc ttatttatgg cgacatgttg tgccacatat
      301 tgagccagta gcgcggtgta ttataccaga ccttattggt atgggcaaat caggcaaatc
      361 tggtaatggt tcttataggt tacttgatca ttacaaatat cttactgcat ggtttgaact
      421 tcttaattta ccaaagaaga tcatttttgt cggccatgat tggggtgctt gtttggcatt
      481 tcattatagc tatgagcatc aagataagat caaagcaata gttcacgctg aaagtgtagt
      541 agatgtgatt gaatcatggg atgaatggcc tgatattgaa gaagatattg cgttgatcaa
      601 atctgaagaa ggagaaaaaa tggttttgga gaataacttc ttcgtggaaa ccatgttgcc
      661 atcaaaaatc atgagaaagt tagaaccaga agaatttgca gcatatcttg aaccattcaa
      721 agagaaaggt gaagttcgtc gtccaacatt atcatggcct cgtgaaatcc cgttagtaaa
      781 aggtggtaaa cctgacgttg tacaaattgt taggaattat aatgcttatc tacgtgcaag
      841 tgatgattta ccaaaaatgt ttattgaatc ggacccagga ttcttttcca atgctattgt
      901 tgaaggtgcc aagaagtttc ctaatactga atttgtcaaa gtaaaaggtc ttcatttttc
      961 gcaagaagat gcacctgatg aaatgggaaa atatatcaaa tcgttcgttg agcgagttct
     1021 caaaaatgaa caagctagcg gaagcggagc cactaactt
//
