LOCUS       sequence-97990-e        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. 
            Contains KrasG12D HDR donor. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre
            -shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(95..170)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(191..439)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(453..528)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(549..797)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(810..885)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcca
       61 attcccactc ctttcaagac ctggtaccaa aaaagcaccg actcggtgcc actttttcaa
      121 gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac gcggcataag
      181 gtctcggagt ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
      241 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
      301 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
      361 tcaaattaat tccaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
      421 tcatgggaaa taggccctct tctagaaaaa aagcaccgac tcggtgccac tttttcaagt
      481 tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaaccc atgcaggagc
      541 tattacacgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
      601 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
      661 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
      721 aaattaattc caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
      781 atgggaaata ggccctcgga tccaaaaaag caccgactcg gtgccacttt ttcaagttga
      841 taacggacta gccttatttt aacttgctat ttctagctct aaaactacga tagaggtaac
      901 gctgcggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      961 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
     1021 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
     1081 ttaattccaa ttatctctct aacagccttg tatcgta
//
