LOCUS       sequence-97995-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of PKN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, 
            Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D
  TITLE     A role for WDR5 in integrating threonine 11 phosphorylation to 
            lysine 4 methylation on histone H3 during androgen signaling and in 
            prostate cancer.
  JOURNAL   Mol Cell. 2014 May 22;54(4):613-25. doi: 
            10.1016/j.molcel.2014.03.043. Epub 2014 May 1.
  PUBMED    24793694
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.043"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "613-25"
COMMENT     Sequence Label: pSuper.Retro.Puro-PKN1 shRNA 4
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     12..28
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(97..311)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(157..176)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(357..380)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gatcgatctc tcgaggtcga cggtatcgat aagcttaaaa acgagagaga tgcagaagat
       61 tctcttgaaa tcttctgcat ctctctcggg ggatctgtgg tctcatacag aacttataag
      121 attcccaaat ccaaagacat ttcacgttta tggtgatttc ccagaacaca tagcgacatg
      181 caaatattgc agggcgccac tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg
      241 cgcgctgggt gttcccgcct agtgacactg ggcccgcgat tccttggagc gggttgatga
      301 cgtcagcgtt cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
      361 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
      421 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
      481 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
      541 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
      601 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
      661 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
      721 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
//
