LOCUS       sequence-98-expo        7254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_27
FEATURES             Location/Qualifiers
     source          1..7254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             527..547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(637..659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4694..4716)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4816..4835
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4913..4931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4999..5103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5104..5172,5173..5964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5322..5341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6135..6723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6624..6643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6877..6894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7011..7032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7047..7052,7053..7070,7071..7077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7090..7112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7109..7125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7109..7125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc cttggtcgac tctagaggat ccccggggat tggccaaagg
       61 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
      121 gcttggctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca
      181 gcatccgcac tggcagtctc cgaaccggcc tgcaggatcg attttttgca aattacgagc
      241 gttgtagggg gcggagcgat aggtcctata ggttttggta tatcatcatt cattcattca
      301 ttggtacatt catttaccca ccttcctctt tctgagcttc tctggagttc tgtgcttcct
      361 ttttccctta tctttatact gtaattttta actttcaggc attgattgga tccccgggat
      421 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      481 gaggaccctt gcttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
      541 taaggtaccg gtgggtgaag accagaaaca gcacctcgaa ctgagccgcg atattgccca
      601 gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt ttacaacgtc gtgactggga
      661 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      781 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
      841 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
      901 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
      961 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     1021 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     1081 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     1141 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct
     1201 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     1261 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     1321 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     1381 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     1441 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     1501 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     1561 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     1621 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     1681 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatgtaag
     1741 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagatcc tgctgatgaa
     1801 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     1861 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     1921 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     1981 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     2041 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     2101 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     2161 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     2221 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     2281 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     2341 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     2401 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     2461 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgt aagtttaaac
     2521 aataacctac taactaacgt agataattta aattttcagg ctgacggaag caaaacacca
     2581 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
     2641 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
     2701 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
     2761 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
     2821 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
     2881 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
     2941 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
     3001 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
     3061 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
     3121 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
     3181 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
     3241 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
     3301 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
     3361 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
     3421 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
     3481 agacatgtag taagtttaaa cttgatagta ctaactaaca tgtttcattt aaattttcag
     3541 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
     3601 tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca
     3661 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
     3721 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     3781 attccaactg agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg
     3841 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat
     3901 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     3961 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     4021 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     4081 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     4141 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     4201 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     4261 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     4321 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     4381 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     4441 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     4501 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     4561 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     4621 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     4681 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4741 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4801 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4861 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     4921 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5641 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6241 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     6541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6781 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6841 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6901 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7141 ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa
     7201 aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
