LOCUS       sequence-98024-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a genomic fragment that is not active in human
            pancreatic islets, a minimal promoter and a luc2 gene. Can be used 
            as negative control in reporter assays performed in human pancreatic
            islets..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23 C5_15
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(619..643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcccgtcga cgaattctgc agatatcaca
       61 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccgacagtat aagtgatttg
      121 tcaggaccac acagccagtg agtgctggag gttagaacag cgggatgtgg cagacctcaa
      181 aagtcagagt cctgatttga aatagatgtt ttaaaagaaa aaaaaataga tacgatattt
      241 actgcagtaa agtctttagg tttcttcaat tggcagctgt aaaaataaaa gcataattct
      301 cacactggtc taattaaaaa cggccaagta caattgttca tggctaatta cctctgtcag
      361 accaaatgga gagtcataca attcatgaga cagtccatta gacctaatta agggaaatta
      421 tttcgagacg actctgaggc aatattagag gatgttttat gctaagattt ctaaaatgtt
      481 ttgtagcaaa tacagcttat gaaccaaagg agaaaccaca aaactgcaac tctgatctag
      541 agcgattcaa acactcagtc tgccccatcc tctcttctcc cctaactcca gggaggggtt
      601 ggaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggtgataaac ccgctgatca
      661 gcctcgactg tgctcgagga tatcaagatc tggcctcggc ggccaagctt agacactaga
      721 gggtatataa tg
//
