LOCUS       sequence-98027-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted SNAP(f)-tag and histone-GFP, 
            non-conditional.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Kohl J, Ng J, Cachero S, Ciabatti E, Dolan MJ, Sutcliffe B, Tozer A,
            Ruehle S, Krueger D, Frechter S, Branco T, Tripodi M, Jefferis GS
  TITLE     Ultrafast tissue staining with chemical tags.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 25. pii: 201411087.
  PUBMED    25157152
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 25. pii: 201411087."
COMMENT     Sequence Label: pAAV-myrSNAP
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..481)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(500..553)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gctcctcgcc cttgctcatg gtggcccgcg gtagcgctag cccacctccg ccgctgcccc
       61 cacctccaga tccacccccg ccggagcctc cacccccaga tcccttagcg ctggtgtact
      121 tggtgacggc cttagtaccc tcggacacgg cgtgcttggc caactcccca ggcagcagca
      181 ggcgcacggc cgtctggatc tccctggagg tgatggtcga gcgcttgttg taatgcgcca
      241 ggcgggaagc ctcacctgcg atgcgctcga aaatgtcgtt cacaaacgag ttcatgatgc
      301 ccatggcctt ggacgaaatg ccggtgtcag ggtggacctg cttcagaacc ttgtacacat
      361 agatggaata gctctccttg cggctgcgct tgcgcttctt gccgcctttc ttctgcgcct
      421 tagtcaccgc cttcttggag ccctttttcg gggcgggagc agacttcgct ggctctggca
      481 tggtgatatt cagatctcct gggccaggat tctcctcgac gtcaccgcat gttagcagac
      541 ttcctctgcc ctctccactg ccacccagcc caggcttgcc cagtctgtgg ccctcgtggg
      601 ccagcagcca ctctttcacg gcgagcccgc cctcgtagcc ccccacgtcc aggtcgccct
      661 gcaccacccg gtggcagggg atcagaatgg gcacgggatt tccgctcagg gcggttttca
      721 cggc
//
