LOCUS       sequence-98035-e         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted SNAP(f)-tag and histone-GFP, 
            conditional.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Kohl J, Ng J, Cachero S, Ciabatti E, Dolan MJ, Sutcliffe B, Tozer A,
            Ruehle S, Krueger D, Frechter S, Branco T, Tripodi M, Jefferis GS
  TITLE     Ultrafast tissue staining with chemical tags.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 25. pii: 201411087.
  PUBMED    25157152
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 25. pii: 201411087."
COMMENT     Sequence Label: pAAV-myrSNAP-CON
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(70..103)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggcactggg gaggggtcac agggatgcca cccgtagatc tctcgagcag cgctgctcga
       61 ggcaagcttt accgttcgta tagcatacat tatacgaagt tatccaccat gggctgcatc
      121 aagagcaagc gcaaggacaa cctgaacgac gacgaggccg ccatgggctg catcaagagc
      181 aagcgcaagg acaacctgaa cgacgacgag gcaccggtcg cctccatgga caaagactgc
      241 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
      301 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
      361 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg
      421 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac
      481 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg
      541 gtgaagttcg gagaggtcat cagctaca
//
