LOCUS       sequence-9804-ex        9981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Yang X, Gregan J, Lindner K, Young H, Kearsey SE
  TITLE     Nuclear distribution and chromatin association of DNA polymerase 
            alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) 
            in fission yeast.
  JOURNAL   BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1186/1471-2199-6-13.
  PUBMED    15941470
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2005-06-7"; volume: "6:13. doi"; pages: " 
            10.1186/1471-2199-6-13"
COMMENT     Sequence Label: pREP3-TEV-NLS
FEATURES             Location/Qualifiers
     source          1..9981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(722..1816)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1817..2224)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3707..3727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3773..3802
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3809..3838
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3857..3886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3893..3922
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3929..3958
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3977..4006
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4013..4042
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4049..4078
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4094..4801
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMSKPE
                     EPFQPVKEATQLMN"
     CDS             4826..4846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4853..4873
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     rep_origin      5977..6760
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7110..7127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7110..7126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7119..7141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7339..7794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7426..7445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7636..7657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7811..7830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7930..7952
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7990..8008)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8076..8180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8181..8249,8250..9041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8399..8418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9212..9800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9701..9720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9954..9971
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 tcgatcgact acgtcgttaa ggccgtttct gacagagtaa aattcttgag ggaactttca
      301 ccattatggg aaatggttca agaaggtatt gacttaaact ccatcaaatg gtcaggtcat
      361 tgagtgtttt ttatttgttg tatttttttt tttttagaga aaatcctcca atatataaat
      421 taggaatcat agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      481 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      541 aatttgctta cctgtattcc tttacatcct cctttttctc cttcttgata aatgtatgta
      601 gattgcgtat atagtttcgt ctaccctatg aacatattcc attttgtaat ttcgtgtcgt
      661 ttctattatg aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt
      721 tttaagcaag gattttctta acttcttcgg cgacagcatc accgacttcc gtggtactgt
      781 tggaaccacc taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt
      841 caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca
      901 agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa ccgtggcatg
      961 gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca
     1021 acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt
     1081 tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag
     1141 aatcaatcaa ttgatgttga accttcaatg tagggaattc gttcttgatg gtttcctcca
     1201 cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag
     1261 gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt
     1321 ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct
     1381 taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa
     1441 attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta
     1501 agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac
     1561 taccggtacc ccatttagga ccacccacag cacctaacaa aacggcatca accttcttgg
     1621 aggcttccag cgcctcatct ggaagtggga cacctgtagc atcgatagca gcaccaccaa
     1681 ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa
     1741 ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct
     1801 tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat attgctgaaa
     1861 tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca
     1921 ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa
     1981 cgcttctcta ttctatatga aaagccggtt ccgcggctct cacctttcct ttttctccca
     2041 atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc
     2101 agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa
     2161 aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac
     2221 agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg gccaaacaac
     2281 caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt ttgaacacac
     2341 atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg atgtaattgt
     2401 tgggattcca tttttaataa ggcaataata ttaggtatgt agatatacta gaagttctcc
     2461 tcgacaagct tgcatgcctg caggtcgatc gactctagag gatcagaaaa ttatcgccat
     2521 aaaagacaga ataagtcatc agcggttgtt tcatttccta tatttttttt ttattttttt
     2581 attttttaat aagggaaaat ttaacgtcta aggatacaga agattgttag cacattaaag
     2641 taataaaggc ttaagtagta agtgccttag catgttattg tatttcaaag gacataatct
     2701 aaaataataa caatatcatt tctcacaagt tattcaattt tctttttttt ttctaataat
     2761 atcaagaatg tattatttgt ttgacataag tcaactaatt tatttaatat gctggattaa
     2821 tcttgcagac atgtaaatta acaagtttta gtcaaataac gttgaagttt caatgaactc
     2881 aaataatttc tctttttttt tatataacca tatgtctaat ctgatttata ttttccgcag
     2941 ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc gccaaataat
     3001 acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc ctctttcttg
     3061 ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc cacaattttg
     3121 ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg acctcaatga
     3181 ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg tacaagtttc
     3241 caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac acttgttata
     3301 tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag taatttcggt
     3361 tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat caggaaaaac
     3421 gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga gaaaacaggg
     3481 caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga aactaaaaac
     3541 cggataatgg acctgttaat cgaaacattg aagatatata aaggaagagg aatcctggca
     3601 tatcatcaat tgaataagtt gaattaatta tttcaatctc attctcactt tctgacttat
     3661 agtcgctttg ttaaatggcc tcgagagaaa aaaccccgga tctatgccaa agaagaagcg
     3721 taaggtcgct agtggtgaac aaaagttagt ttctgaagaa gatttggacg gtgaacaaaa
     3781 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     3841 cggatcctct agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     3901 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     3961 cggatcctct agaggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     4021 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     4081 cggatccact agcggagaaa gcttgtttaa gggaccacgt gattacaacc cgatatcgag
     4141 caccatttgt catttgacga atgaatctga tgggcacaca acatcgttgt atggtattgg
     4201 atttggtccc ttcatcatta caaacaagca cttgtttcgt cgcaataatg gaacactgtt
     4261 ggtccaatca ctacatggtg tattcaaggt caagaacacc acgactttgc aacaacacct
     4321 cattgatggg agggacatga taattattcg catgcctaag gatttcccac catttcctca
     4381 aaagctgaaa tttagagagc cacaaaggga agagcgcata tgtcttgtga caaccaactt
     4441 ccaaactaag agcatgtcta gcatggtgtc agacactagt tgcacattcc cttcatctga
     4501 tggcatattc tggaagcatt ggattcaaac caaggatggg cagtgtggca gtccattagt
     4561 atcaactaga gatgggttca ttgttggtat acactcagca tcgaatttca ccaacacaaa
     4621 caattatttc acaagcgtgc cgaaaaactt catggaattg ttgacaaatc aggaggcgca
     4681 gcagtgggtt agtggttggc gattaaatgc tgactcagta ttgtgggggg gccataaagt
     4741 tttcatgagc aaacctgaag agccttttca gccagttaag gaagcgactc aactcatgaa
     4801 tgaattggtg tactcgcaag ggtcacctaa gaaaaaacga aaagttgagg atcctaaaaa
     4861 gaaacgaaaa gttgattaac tgcaggcatg caagcttggc cccgggtaaa ggaatgtctc
     4921 ccttgccagt actgctaggg tttttctttc aaactatgga agcccattca agctgcatat
     4981 tacgattttg tttttcgctt ttagaaagtg gtttagatga gataatagaa aaattcttga
     5041 tctccgacaa cgagtacttt tatttttttt gctaatcact ttactcaata ttagctcgaa
     5101 atcgtagaaa cgtagacggg tgcgggatac cgagtggtgt agttaagaat ttttataaac
     5161 cacgtggccc aaaaatatga acccaaaacg tttatacatg agtatacttt aagaaggcta
     5221 taccccttcg tgttagatgt agttttagct acccaacccg agtctatgag cttgacttca
     5281 gatgtagaag gcattaaatc gttttgaata ttaattaaaa aacgatgaaa attaaatatt
     5341 taaaagcaat catacgctga aaatttagtg ctgtggctaa tccttcaaca tggaaatgcc
     5401 ataaaagtga ctttgacaaa aaaaaaagta tatacaggta gtaaactcat ctacttcatt
     5461 gactttgttt acagcatgtg gaaggaggaa tatttattgc taaatcgtag tttaacattc
     5521 aataagtaat actattgaaa ttcgacaaga ttggccgcat ggatgaaaaa gaggcatttt
     5581 gctttgggag aattagttca aattagaact gaaaaaaaaa actttacgag gcaaaaatgt
     5641 cggattgaga tcgtaaaagt tcgctcgtcg tcttttgctt tgtgattgtt ttcatggata
     5701 catcttgctg gatatttaaa ttttagtact atgtataaga tattctataa atgttttatc
     5761 acccaaacct gttagcgcct tcttaattct attcaatctg gcttttgctc tgagactact
     5821 tcttggactt tcactacttg ttagttatac ggaatttgtg taattagaag tgaaataatc
     5881 ctttctatta gtaatgcgag ctcgaattcg agtctaactc cttaaccact cggacatagt
     5941 gacttatctg acactcattg taaattaata tatatatagg cattttgttt agttaaaggt
     6001 acttaagtaa ttagtataaa cgaaccaatt ttataatcag gaagttaagt gaatggtagc
     6061 acatgtcgta aaaattgtga atttttattg aataatattt taaatacaag cctttctaga
     6121 ctaggtatac tcataaacat atatgagcaa aaggatagag gagattacat tgcatcttct
     6181 acaaatttat ttattgccct ttactgaaaa attaaataat gagtactaaa tgataaaaag
     6241 cgctcagtac aagaaagatt gcaaaaatat tgcattcttc atgaattaaa gttgcatata
     6301 aggcatattg aaagtaatag tactaaaaca gcagttagcg aaaattaata gaattatatt
     6361 cgcaagacaa ttgtgacatt aaattaaaaa attgtagaat ttttactatc ctctttaacg
     6421 ccatgagcct ttataaaaag gttaaattag ttttaacatt ctttttttga gtaagagttt
     6481 acaatttatc aaaacctgtg ttattatatt cattagtttc aatttattag catctagaga
     6541 aaaatcaatt ggcagttaca ttgttggaat ttatgaagaa aaagaatcta caacggagaa
     6601 taagttgctg atcgctttcc ctaaaattgt atattttgct gagcttattt tgacatttcg
     6661 ttgaagtttt ctctaattcg cattcatttt aagtaaaaca atgagaaata aaattacaaa
     6721 aaatacaaat taaaatacaa tttttagcta taattataga cgatgccctt gtatcccatt
     6781 ctgtctcgct tgcccctact ttttatcttt tatataccat aatgaacgct gccgctacta
     6841 accatacccc gattttacat ttcggactcc caaggacgta caaaatagaa aactatagaa
     6901 aaaaataatc agaaaatagc atgtcatctc tttgtaaaac gcgtttgcaa gaagaaagga
     6961 aacaatggag aagagatcat ccatttgtat gtaaatttta gtaaacttga agaaatcact
     7021 aacaacttct cttacttagg gattctatgc aaaaccttgt aaatcatctg atggaggact
     7081 cgatttaatg aattggaagg ttggaattca ctggccgtcg ttttacaacg tcgtgactgg
     7141 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7201 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7261 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7321 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     7561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7681 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7741 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     7801 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7861 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7921 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7981 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     8041 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     9361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9961 cagtgagcga ggaagcggaa g
//
