LOCUS       sequence-98058-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry
            that can be used to degrade R-loops. Confers resistance to 
            Puromycin. Expression is inducible in T-REx cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B,
            Frit P, Calsou P
  TITLE     DNA damage triggers SAF-A and RNA biogenesis factors exclusion from 
            chromatin coupled to R-loops removal.
  JOURNAL   Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 
            10.1093/nar/gku601. Epub 2014 Jul 16.
  PUBMED    25030905
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku601"; journalName: "Nucleic Acids Res"; date: 
            "2014-10-1- 1"; volume: "42"; issue: "14"; pages: "9047-62"
COMMENT     Sequence Label: pICE-RNaseHI-WT-NLS-mCherry
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     30..54
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             605..625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             638..658
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      668..677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(819..837)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
       61 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      121 caagcttgcc accatgctga aacaggtgga aatcttcacc gacggcagct gtctgggcaa
      181 tcctggccct ggcggatacg gcgccatcct gagataccgg ggcagagaga aaaccttcag
      241 cgccggctac acccggacca ccaacaaccg gatggagctg atggccgcca tcgtggccct
      301 ggaagccctg aaagaacact gcgaagtgat cctgagcacc gacagccagt acgtgcggca
      361 gggcatcacc cagtggattc acaactggaa gaagcggggc tggaaaaccg ccgacaagaa
      421 acccgtgaag aacgtggacc tgtggcagag actggatgcc gccctgggac agcaccagat
      481 caagtgggag tgggtcaagg gccacgccgg ccaccctgag aatgagagat gtgacgagct
      541 ggccagagcc gccgctatga accccaccct ggaagatacc ggctaccagg tggaagtggg
      601 cagccccaag aaaaagcgga aagtgggcac gcgtggcccc aaaaagaaac gcaaagtggg
      661 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      721 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      781 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      841 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      901 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      961 caagtggga
//
