LOCUS       sequence-98064-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Kim J, Zhang CZ, Zhang X, Springer TA
  TITLE     A mechanically stabilized receptor-ligand flex-bond important in the
            vasculature.
  JOURNAL   Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295.
  PUBMED    20725043
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-19- 19"; volume: "466"; issue: "7309"; 
            pages: "992-5"
COMMENT     Sequence Label: ReaLiSM A1-26link-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..129)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccacaccagc caccaccttc tgataggcag cctgcacctg aggagtgaat tatcgcgata
       61 ctagtctcga ggcatgcgcg gccgcggatc cggcgcgcca ctagtttacg cgtgatggtg
      121 atggtgatga ccgcagccct tggtaccacg caccttatcg ccctcagtgt cctcttctgg
      181 gtagtaatca tataggtctg tgtcaccttc atcaccaagg gtggggcacc cctttcctgg
      241 gtatttgtag acgggaaact tgtctgaatt gtcacactgc acactggcca cgttagaggt
      301 catggccttg acgtccacac cttgcttcca tacgtagaca ttttcagcat tgtcctgcag
      361 ccagcgacga aaatagagga tctcacagtt gcataaccag gggttcccgt ggagaaaagc
      421 aaaaggcagg aggtgggacc caaaaaagcc ctttggtatt gtatacagcg agttctcttg
      481 gaggagaagg gtgtcgagat tctccagccc attcaggagc ccagcgggga gctcagtcaa
      541 gttgttgtta gccagactga gcttctccag cttgggtgtg ggcgtcagga gccctggggg
      601 cagggtcttc agctcattgc ctttcaggta gagctcttgg agttcgccaa gaccacgcag
      661 ggcaccaaga ggcagcgagg tcagccggtt gaaggagacg tccaggacg
//
