LOCUS       sequence-98075-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 kb zebrafish insulin promoter driving eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   diIorio PJ, Moss JB, Sbrogna JL, Karlstrom RO, Moss LG
  TITLE     Sonic hedgehog is required early in pancreatic islet development.
  JOURNAL   Dev Biol. 2002 Apr 1;244(1):75-84.
  PUBMED    11900460
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2002-04-1"; volume: "244(1)"; pages: "75-84"
COMMENT     Sequence Label: pKSII/INSeGFP
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..226
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     218..235
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..264
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        245..263
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    435..569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(472..491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     526..545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(659..680)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
      121 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
      181 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
      241 cgcgtaatac gactcactat agggcgaatt ggagctcgaa ttcggcttaa gatatctcga
      301 gcactataga accagatctg atctagagga tcataatcag ccataccaca tttgtagagg
      361 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      421 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      481 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      541 tcatcaatgt atcttatcat gtctggatca tcatcgatga attaattcga gctccaccgc
      601 ggtggcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      661 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      721 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      781 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      841 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
      901 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
      961 cgatgcggtt caccagggtg tcgccctcga acttcacctc g
//
