LOCUS       sequence-98085-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a genomic fragment that is not active in human
            pancreatic islets, a minimal promoter and a luc2 gene. Can be used 
            as negative control in reporter assays performed in human pancreatic
            islets..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pGL4.23 C5_14
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    214..335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(251..270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     305..324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
       61 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacggat ccttatcgat
      121 tttaccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      181 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      241 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      301 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgctcgaa gcggccggcc
      361 gccccgactc tagaattatt acacggcgat cttgccgccc ttcttggcct taatgagaat
      421 ctcgcggatc ttgcgggcgt ccaacttgcc ggtcagtcct ttaggcacct cgtccacgaa
      481 cac
//
