LOCUS       sequence-98086-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses wildtype alpha-synuclein 
            under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Savolainen MH, Richie CT, Harvey BK, Mannisto PT, Maguire-Zeiss KA, 
            Myohanen TT
  TITLE     The beneficial effect of a prolyl oligopeptidase inhibitor, 
            KYP-2047, on alpha-synuclein clearance and autophagy in A30P 
            transgenic mouse.
  JOURNAL   Neurobiol Dis. 2014 Aug;68:1-15. doi: 10.1016/j.nbd.2014.04.003. 
            Epub 2014 Apr 16.
  PUBMED    24746855
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis."; date: "2014-08"; volume: "68:1-15. doi"; pages: " 
            10.1016/j.nbd.2014.04.003. Epub 2014 Apr 16"
COMMENT     Sequence Label: pOTTC293 - pAAV EF1a V5-synuclein (WT)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..72
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(611..631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tttcaggtgt cgtgaggtac cggatccatg ggtaagccta tccctaaccc tctcctcggt
       61 ctcgattcta cggctagcat ggatgtattc atgaaaggac tttcaaaggc caaggaggga
      121 gttgtggctg ctgctgagaa aaccaaacag ggtgtggcag aagcagcagg aaagacaaaa
      181 gagggtgttc tctatgtagg ctccaaaacc aaggagggag tggtgcatgg tgtggcaaca
      241 gtggctgaga agaccaaaga gcaagtgaca aatgttggag gagcagtggt gacgggtgtg
      301 acagcagtag cccagaagac agtggaggga gcagggagca ttgcagcagc cactggcttt
      361 gtcaaaaagg accagttggg caagaatgaa gaaggagccc cacaggaagg aattctggaa
      421 gatatgcctg tggatcctga caatgaggct tatgaaatgc cttctgagga agggtatcaa
      481 gactacgaac ctgaagccta agaaatatct ttgctcccag tttcttgaga tctgaattcg
      541 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
      601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      901 gg
//
