LOCUS       sequence-98087-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses wildtype alpha-synuclein 
            under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Savolainen MH, Richie CT, Harvey BK, Mannisto PT, Maguire-Zeiss KA, 
            Myohanen TT
  TITLE     The beneficial effect of a prolyl oligopeptidase inhibitor, 
            KYP-2047, on alpha-synuclein clearance and autophagy in A30P 
            transgenic mouse.
  JOURNAL   Neurobiol Dis. 2014 Aug;68:1-15. doi: 10.1016/j.nbd.2014.04.003. 
            Epub 2014 Apr 16.
  PUBMED    24746855
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis."; date: "2014-08"; volume: "68:1-15. doi"; pages: " 
            10.1016/j.nbd.2014.04.003. Epub 2014 Apr 16"
COMMENT     Sequence Label: pOTTC293 - pAAV EF1a V5-synuclein (WT)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(525..566)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          591..794
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(614..634)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa
       61 ttcagatctc aagaaactgg gagcaaagat atttcttagg cttcaggttc gtagtcttga
      121 tacccttcct cagaaggcat ttcataagcc tcattgtcag gatccacagg catatcttcc
      181 agaattcctt cctgtggggc tccttcttca ttcttgccca actggtcctt tttgacaaag
      241 ccagtggctg ctgcaatgct ccctgctccc tccactgtct tctgggctac tgctgtcaca
      301 cccgtcacca ctgctcctcc aacatttgtc acttgctctt tggtcttctc agccactgtt
      361 gccacaccat gcaccactcc ctccttggtt ttggagccta catagagaac accctctttt
      421 gtctttcctg ctgcttctgc cacaccctgt ttggttttct cagcagcagc cacaactccc
      481 tccttggcct ttgaaagtcc tttcatgaat acatccatgc tagccgtaga atcgagaccg
      541 aggagagggt tagggatagg cttacccatg gatccggtac ctcacgacac ctgaaatgga
      601 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
      661 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
      721 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
      781 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
      841 catgaagcga cg
//
