LOCUS       sequence-98101-e        1890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule fused to dockerin type III 
            domain to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced Cystein in Xylanase T120C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-HIS-Xyl-Xmod-DocIII
FEATURES             Location/Qualifiers
     source          1..1890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagccacc atcaccatca ccataagaat gcagattcct atgcgaaaaa acctcacatc
       61 agcgcattga atgccccaca attggatcaa cgctacaaaa acgagttcac gattggtgcg
      121 gcagtagaac cttatcaact acaaaatgaa aaagacgtac aaatgctaaa gcgccacttc
      181 aacagcattg ttgccgagaa cgtaatgaaa ccgatcagca ttcaacctga ggaaggaaaa
      241 ttcaattttg aacaagcgga tcgaattgtg aagttcgcta aggcaaatgg catggatatt
      301 cgcttccata cactcgtttg gcacagccaa gtacctcaat ggttctttct tgacaaggaa
      361 ggtaagccaa tggttaatga atgcgatcca gtgaaacgtg aacaaaataa acaactgctg
      421 ttaaaacgac ttgaaactca tattaaaacg atcgtcgagc ggtacaaaga tgacattaag
      481 tactgggacg ttgtaaatga ggttgtgggg gacgacggaa aactgcgcaa ctctccatgg
      541 tatcaaatcg ccggcatcga ttatattaaa gtggcattcc aagcagctag aaaatatggc
      601 ggagacaaca ttaagcttta catgaatgat tacaatacag aagtcgaacc gaagcgaacc
      661 gctctttaca atttagtcaa acaactgaaa gaagagggtg ttccgatcga cggcatcggc
      721 catcaatccc acatccaaat cggctggcct tctgaagcag aaatcgagaa aacgattaac
      781 atgttcgccg ctctcggttt agacaaccaa atcactgagc ttgatgtgag catgtacggt
      841 tggccgccgc gcgcttaccc gacgtatgac gccattccaa aacaaaagtt tttggatcag
      901 gcagcgcgct atgatcgttt gttcaaactg tatgaaaagt tgagcgataa aattagcaac
      961 gtcaccttct ggggcatcgc cgacaatcat acgtggctcg acagccgtgc ggatgtgtac
     1021 tatgacgcca acgggaatgt tgtggttgac ccgaacgctc cgtacgcaaa agtggaaaaa
     1081 gggaaaggaa aagatgcgcc gttcgttttt ggaccggatt acaaagtcaa acccgcatat
     1141 tgggctatta tcgaccacaa agtggtacca aacactgtta catcagctgt aaagacacag
     1201 tatgttgaga tcgaatctgt tgacggcttc tacttcaaca cagaagacaa gttcgataca
     1261 gctcagatca agaaggctgt tctccacaca gtttataacg aaggctacac aggtgatgac
     1321 ggcgtggctg tagttctcag agaatatgag agcgagcctg ttgatatcac agctgagtta
     1381 acattcggtg atgcaacacc tgctaacaca tacaaggctg ttgaaaacaa gttcgattac
     1441 gagatccctg tttactacaa caacgctaca ctcaaggatg ctgagggcaa cgatgctaca
     1501 gtaactgttt acatcggtct caagggcgat acagacctta acaacatcgt tgacggtcgt
     1561 gacgctacag caacacttac ttactacgct gcaacatcta cagacggcaa ggatgctaca
     1621 acagttgcac tttcaccaag cacactggtt ggcggcaacc ctgagtcagt atatgatgac
     1681 ttctctgcat tccttagcga tgttaaggta gacgctggta aggaactcac aagattcgct
     1741 aagaaggctg agagacttat cgacggtaga gatgcttcaa gcatactcac attctacact
     1801 aagtcttctg tagatcagta caaggatatg gctgctaacg agccaaacaa gctttgggat
     1861 atcgttacag gcgatgctga agaagaataa
//
