LOCUS       sequence-98110-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutated HNF4A P2 promoter region cloned into the pGL2-basic 
            backbone. The -181 G to A mutation is in a HNF1alpha binding site 
            and cosegregates with MODY..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Hansen SK, Parrizas M, Jensen ML, Pruhova S, Ek J, Boj SF, Johansen 
            A, Maestro MA, Rivera F, Eiberg H, Andel M, Lebl J, Pedersen O, 
            Ferrer J, Hansen T
  TITLE     Genetic evidence that HNF-1alpha-dependent transcriptional control 
            of HNF-4alpha is essential for human pancreatic beta cell function.
  JOURNAL   J Clin Invest. 2002 Sep;110(6):827-33.
  PUBMED    12235114
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2002-09"; volume: "110(6)"; pages: "827-33"
COMMENT     Sequence Label: pGL2 HNF4A P2 mut
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(451..470)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     505..524
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatttt accaacagta ccggaatgcc
       61 aagcttactt agatctcgag cacagcagca gcacagccac caggagcccg tccaccagga
      121 aggcggtgag tgcggccagc tgccaccgcc cggttatctt attgattctt ctaatcaccc
      181 aaggtgggtg gatacgttaa agagtaatca gtcacttagg gaacccgcgg ctggggactg
      241 ttgctgtctc catggcaatg gcgacctggc cagacctgag gtccactcgg gtcctagtcc
      301 tgcactctgg ggttagggga acccagagcc aggtgtatgg ggacatcagc cccccaattt
      361 gaggacaggg ccggagcaga aggggctgga gaggtgagct cggtacctcc cgggttatgt
      421 tagctcagtt acagtaccat aagatacatt gatgagtttg gacaaaccac aactagaatg
      481 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      541 ataagctgca ataaaca
//
