LOCUS       sequence-98112-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. For expression of transgenes
            under a CMV promoter when a GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack-CMV
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    200..321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(211..230)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     265..284
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(502..523)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaactgaata agaggaagtg aaatctgaat aattttgtgt tactcatagc gcgtaatatt
       61 tgtctagggc cgcggggact ttgaccgttt acgtggagac tcgcccaggt gtttttctca
      121 ggtgttttcc gcgttccggg tcaaagttgg cgttttatta ttatagtcag tcgagtctag
      181 caagctagct tgatcgcgtt aagatacatt gatgagtttg gacaaaccac aactagaatg
      241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      361 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
      421 tatgatcagt tatctagatc cggtggatct gagtccggac ttgtacagct cgtccatgcc
      481 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      541 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      601 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      661 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      721 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      781 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      841 ctcggcgcgg gtcttg
//
