LOCUS       sequence-98129-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Labels synaptic vesicles for electron microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Atasoy D, Betley JN, Li WP, Su HH, Sertel SM, Scheffer LK, Simpson 
            JH, Fetter RD, Sternson SM
  TITLE     A genetically specified connectomics approach applied to long-range 
            feeding regulatory circuits.
  JOURNAL   Nat Neurosci. 2014 Dec;17(12):1830-9. doi: 10.1038/nn.3854. Epub 
            2014 Nov 2.
  PUBMED    25362474
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3854"; 
            journalName: "Nat Neurosci"; date: "2014-12"; volume: "17"; issue: 
            "12"; pages: "1830-9"
COMMENT     Sequence Label: AAV-FLEX-VAMP2:HRP
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..85
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    180..213
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    284..317
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
       61 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
      121 gtaatccaga ggttgattat cgataagctt gatatcgaat tcgaacctct tcgagggaca
      181 taacttcgta taatgtatgc tatacgaagt tatgggtcga tggtgagatc tggactagag
      241 ggtcgatggt gatgcttgga tcaaaatagg aagaatccca atgataactt cgtataaagt
      301 atcctatacg aagttatact aggatccatg tcggctaccg ctgccaccgc cccccctgcc
      361 gccccggctg gggagggagg ccctcctgcg ccccctccaa acctcactag taacaggaga
      421 ctgcagcaga cccaggccca ggtggatgag gtggtggaca tcatgagggt gaacgtagac
      481 aaggtcttgg agcgggacca gaagctgtcg gagctggacg accgtgccga tgccctccag
      541 gcgggggcct cccagtttga aactagtgca gccaagctca agcgcaaata ctggtggaaa
      601 aacctcaaga tgatgatcat cctgggagtg atttgcgcca tcatcctcat tatcatcatc
      661 gtttacttca gcactgccgg cggctccggc ggcaccggcg gctccggcgg caccgagcag
      721 aagctgatct ccga
//
