LOCUS       sequence-98131-e        9629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses DLX1 under the
            EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Victor MB, Richner M, Hermanstyne TO, Ransdell JL, Sobieski C, Deng 
            PY, Klyachko VA, Nerbonne JM, Yoo AS
  TITLE     Generation of Human Striatal Neurons by MicroRNA-Dependent Direct 
            Conversion of Fibroblasts.
  JOURNAL   Neuron. 2014 Oct 22;84(2):311-23. doi: 10.1016/j.neuron.2014.10.016.
            Epub 2014 Oct 22.
  PUBMED    25374357
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.10.016"; journalName: "Neuron"; date: 
            "2014-10-22- 22"; volume: "84"; issue: "2"; pages: "311-23"
COMMENT     Sequence Label: phDLX1-N174
FEATURES             Location/Qualifiers
     source          1..9629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4207..4706
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4242..4265)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4639..4658
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4733..5527
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4733..5527
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4787..4806)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5397..5416
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5551..6139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5604..5624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6022..6033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6347..6980
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7109..7125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7109..7125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7122..7144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7157..7163,7164..7181,7182..7187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7340..7357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7511..8096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7591..7610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8267..9058,9059..9127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8890..8909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9128..9232)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9280..9414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9317..9336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9371..9390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccgccaccat gaccatgacc accatgccag
     3421 aaagtctcaa cagccccgtg tcgggcaagg cggtgtttat ggagtttggg ccgcccaacc
     3481 agcaaatgtc tccttctccc atgtcccacg ggcactactc catgcactgt ttacactcgg
     3541 cgggccattc gcagcccgac ggcgcctaca gctcagcctc gtccttctcc cgaccgctgg
     3601 gctaccccta cgtcaactcg gtcagcagcc acgcatccag cccctacatc agttcggtgc
     3661 agtcctaccc gggcagcgcc agcctcgccc agagccgcct ggaggaccca ggggcggact
     3721 cggagaagag cacggtggtg gaaggcggtg aagtgcgctt caatggcaag ggaaaaaaga
     3781 tccgtaaacc caggacgatt tattgcagtt tgcagttgca ggctttgaac cggaggttcc
     3841 agcaaactca gtacctagct ctgccggaga gggcggagct cgcggcctct ttgggactca
     3901 cacagactca ggtcaagatc tggttccaaa acaagcgatc caagttcaag aagctgatga
     3961 agcagggtgg ggcggctctg gagggtagtg cgttggccaa cggtcgggcc ctgtctgctg
     4021 gctccccacc cgtgccgccc ggctggaacc ctaactcttc atccgggaag ggctcaggag
     4081 gaaacgcggg ctcctatatc cccagctaca catcgtggta cccttcagcg caccaagaag
     4141 ctatgcagca accccaactt atgtgaggcc gcgccggctc tagatcgcga acgcgtgaat
     4201 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4261 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4321 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4381 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4441 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4501 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4561 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4621 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     4681 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttcctagga ccatgattga
     4741 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4801 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4861 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4921 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4981 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5041 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5101 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5161 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5221 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5281 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5341 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5401 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5461 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5521 cttctgaccc gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     5581 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5641 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5701 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5761 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5821 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5881 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5941 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6001 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6061 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6121 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     6181 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     6241 aggaggagga ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6301 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     6361 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6421 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6481 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     6541 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     6601 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     6661 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     6721 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     6781 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6961 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7021 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7081 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8041 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8581 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8881 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9241 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
     9301 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9361 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     9421 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     9481 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9541 aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt
     9601 tgttaaatat gtactacaaa cttagtagt
//
