LOCUS       sequence-98158-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green click beetle luciferase from Promega driven by MET17 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB768
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             741..749
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 cttatttgta caaaccgccg gccttctcca acaattgttt caacagctcc ttgcgggtaa
      121 ttttgcctgt tacgttacga gggatggagt caacaaaacg cacgccgcca cgcaagtact
      181 tagtatggct cacacgttca gccaggtaat catacacttc tttggcggta atttctttac
      241 caggctgctt gacaacgaaa gcagaaggca gttcgccggc ctccagatca ggaatgccga
      301 ccacagcgac atcgcgaatg catggatttt tcaacagaat ctcctccaac tcagctggag
      361 caacctggct acccttgtat ttgatcagct ccttgtaacg atccacgacg taaaaatgct
      421 catcttcgtc gtaatatcca aaatcaccag aatgcaacca gccgtcgtcg tcgatggcct
      481 ccttggtagc ttcaacgtta ttgacataac ccttgctcac catagggcct ttgatacaca
      541 gctcgcccac ttggttcggg cccaaagcct taccagtttc gcgatcagcg atcttagcag
      601 ccatgagtgg agtgacacgg cccaaagagc cgctcttaaa ctcatccccg agagagtgaa
      661 tgttagcgct ggtagattcg gtgaggccga agccacaacg aatccctgga agattcaagc
      721 gtttggcggc cacttcagcg acctccttag ccagtggagc ggcaccgcaa cacaattcac
      781 gcagtgaaga caagtcgtac ttgtccacga gtgggctctt agacaaaaac aggatcacgc
      841 taggcacgtt gatgacactg cggacttcat aatcttggat ggctttcaag aaagcctcct
      901 gatcaaaacg gcggaacata atcacgcgg
//
