LOCUS       sequence-98159-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak 
            mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre 
            attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK331
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagtgaggat ttcggtttgc atacccggga aaacttgaac agccgcgcta tagcaccaat
       61 gatgctttcg ctggccctgt cagcgtaacc ataacctatg ctgacacagg ttccgccggg
      121 attcagatgc agacaagctt tcttggtcaa catgctaagc ttaatggcat ggtcttcaca
      181 ctgctgatag tgatggtatt tatatggggt cctcacatta acaaatatta tgtcatattt
      241 gggcacatca cctgggatgc ctaaatccag ccgagctctg aaggtagcct caggccggtc
      301 tgacaaccag tcaaccattt tgcctgggac ggacaacttt tccccgacca ccaggacagt
      361 tctgcccttc aatttgctga cgaatgaaga aaagtcactc tgtgggtgtt cattatggtg
      421 gaggactaaa gcatgaggca gtcttctgtt tacaggtact aggtttatgc gcggatcata
      481 attgcgcagt gtaccagtgt tcatgtcata gactcttcca gtggcaactg cccgaggcag
      541 ttgtgggtac ctgcgagaga gctgacggac cacttcttta ttcagcccgt acatgttagg
      601 cgacggggag ttatcccagt gattattcct aatggataac ggaacagtgg gtgcagaaaa
      661 tagaccggag tccagatcga gtccaaagaa cctcacgcat agttggttca atactatctc
      721 tgctgagtga gctttgtccg tttcaaaata atccacagtg ttccattgtt cagtggtcat
      781 gtctatgcca gcggtcttca gcaccggcac taa
//
