LOCUS       sequence-98169-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB776
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgggctaat tatggggtgt cgcccttcgc tgaatttaat taaccatggc tccaccaaaa
       61 aaaaaaagaa aagttgccat gggtgggggc agcatggaag aagaaaacat tgtgaatgga
      121 gatcgtcctc gtgatctagt ttttcctggc acagcaggac tacaattata tcaatcatta
      181 tataaatatt catatattac tgacggaata atcgatgccc ataccaatga agtaatatca
      241 tatgctcaaa tatttgaaac cagctgccgc ttggcagtta gtctagaaaa atatggcttg
      301 gatcataaca atgttgtggc aatatgcagt gaaaacaaca tacacttttt tggcccttta
      361 attgctgctt tataccaagg aataccaatg gcaacatcaa atgatatgta tacagaaagg
      421 gagatgattg gccatttgaa tatatcgaaa ccatgcctta tgttttgttc aaagaaatca
      481 ctcccattta ttctgaaagt acaaaaacat ctagatttcc ttaaaaaagt catagtcatt
      541 gatagtatgt acgacatcaa tggcgttgaa tgcgtattta gctttgtttc acgttatact
      601 gatcacgcct ttgatccagt gaaatttaac ccaaaagagt ttgatccctt ggaaagaacc
      661 gcattaatta tgacatcatc tggaacaact ggattgccta aaggggtagt aataagccat
      721 agaagtataa ctataagatt cgtccatagc agtgatccca tctatggtac tcgtattgct
      781 ccagatacat caattcttgc tatagcaccg ttccatcatg cctttggact gtttactgca
      841 ctagcttact ttccagtagg acttaagatt gtaatggtga agaaatttga gggcgaattc
      901 ttcttaaaaa ccatacaaaa ttacaaaatc gcttctattg tagttcctcc tccaattatg
      961 gtatatttgg ctaaaagtcc attagtcgat gaat
//
