LOCUS       sequence-98179-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green click beetle luciferase (Promega) driven by LEU1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB778
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             742..750
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag aagtggcgcg
       61 ccttatttgt acaaaccgcc ggccttctcc aacaattgtt tcaacagctc cttgcgggta
      121 attttgcctg ttacgttacg agggatggag tcaacaaaac gcacgccgcc acgcaagtac
      181 ttagtatggc tcacacgttc agccaggtaa tcatacactt ctttggcggt aatttcttta
      241 ccaggctgct tgacaacgaa agcagaaggc agttcgccgg cctccagatc aggaatgccg
      301 accacagcga catcgcgaat gcatggattt ttcaacagaa tctcctccaa ctcagctgga
      361 gcaacctggc tacccttgta tttgatcagc tccttgtaac gatccacgac gtaaaaatgc
      421 tcatcttcgt cgtaatatcc aaaatcacca gaatgcaacc agccgtcgtc gtcgatggcc
      481 tccttggtag cttcaacgtt attgacataa cccttgctca ccatagggcc tttgatacac
      541 agctcgccca cttggttcgg gcccaaagcc ttaccagttt cgcgatcagc gatcttagca
      601 gccatgagtg gagtgacacg gcccaaagag ccgctcttaa actcatcccc gagagagtga
      661 atgttagcgc tggtagattc ggtgaggccg aagccacaac gaatccctgg aagattcaag
      721 cgtttggcgg ccacttcagc gacctcctta gccagtggag cggcaccgca acacaattca
      781 cgcagtgaag acaagtcgta cttgtccacg agtgggctct tagacaaaaa caggatcacg
      841 ctaggcacgt tgatgacact gcggacttca taatcttgga tggctttca
//
