LOCUS       sequence-98185-e        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion driven by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB655
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..779)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDESRE"
ORIGIN
        1 aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag aagtggcgcg
       61 ccttattctc gagattcatc aataccatgg gtaataccag cagcagtaac aaattctaac
      121 aagaccatgt ggtctctctt ttcgtttgga tctttggata aggcagattg ataggataag
      181 taatggttgt ctggtaacaa gactggacca tcaccaattg gagtattttg ttgataatgg
      241 tcagctaatt gaacaccacc atcttcaatg ttgtgtctaa ttttgaagtt agctttgata
      301 ccattctttt gtttgtcagc agtgatgtaa acattgtgag agttatagtt gtattccaat
      361 ttgtgaccta aaatgttacc atcttcttta aaatcaatac cttttaattc gattctatta
      421 actaaggtat caccttcaaa cttgacttca gctctggtct tgtagttacc gtcatctttg
      481 aaaaaaatag ttctttcttg aacataacct tctggcatgg cagacttgaa aaagtcatgt
      541 tgtttcatat gatctgggta tctagcaaaa cattgcaaac cataacctaa agtagtgact
      601 aaggttggcc atggaactgg caatttacca gtagtacaaa tcaattttaa ggtcaattta
      661 ccgtaagtag catcaccttc accttcaccg gagacagaaa atttgtgacc attaacatca
      721 ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc tttagacatt
      781 gatcccccac ccacggcgat ctttccgccc ttcttggcct ttatgaggat ctctctgatt
      841 tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa cacaactcct
      901 ccgcgcaact ttttcgcggt tgttacttga ccggcgacgt aatccacgat ctctttttcc
      961 gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg gaagttca
//
