LOCUS       sequence-98186-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA), driven by LEU1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB756
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctctgatgaa
       61 cggtaagcca atgaaggaaa agaagtctac cagaagtgtc gaagacttca tggctgctat
      121 gcaaagagtc tctactcaac acgaaaagga aacctaatcc tgttgaagta gcatttaatc
      181 ataatttttg tcacatttta atcaacttga tttttctggt ttaatttttc taattttaat
      241 tttaattttt ttatcaatgg gaactgatac actaaaaaga attaggagcc aacaagaata
      301 agccgcttat ttcctactag agtttgctta aaatttcatc tcgaattgtc attctaatat
      361 tttatccaca cacacacctt aaaattttta gattaaatgg catcaactct tagcttcaca
      421 cacacacaca caccgaagct ggttgtttta tttgatttga tataattggt ttctctggat
      481 ggtacttttt ctttcttggt tatttcctat tttaaaatat gaaacgcaca caagtcataa
      541 ttattctaat agagcacaat tcacaacacg cacatttcaa ctttaatatt tttttagaaa
      601 cactttattt agtctaattc ttaattttta atatatataa tgcacacaca ctaatttatt
      661 cattaatttt ttattgagta ggatttgaaa atatttggta tctttgcaag atgtttgtat
      721 agagggacaa agaatcgtct ttattatggt caaggcttta cgtcataata gttcctgccc
      781 agctcttcta taatacttta aagatctctt ctcgtttgct ccatttggaa gtctcgc
//
