LOCUS       sequence-98192-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green beetle luciferase with nuclear localization signal (NLS) and 
            adjustable degron tag (ssrA), driven by LYS9 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB754
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcaagaagc
       61 ccacgagcaa taatcctcct ggcatattta ctaatggaac acgttctcat agaaagcaac
      121 ccctaaaaag aaaggactag tcaaccacaa gccttttaaa aagaaaaatt gaaaaaataa
      181 taaaaaaatt tgaaaaagaa aaaaaaaatc ataaaaccaa tcttaagaaa aaccaaaaaa
      241 aaaccgctaa ttaaaatcta aaaaattgaa acaagaaact gtatgattag taactaatat
      301 actggcgcgg aagggaatga atgccattta aatgagatga ttatgaaaat atagtgtaga
      361 gtagatgcgc gaaaccgagg cagaaaagaa aaagagacaa gttaaaaatt gaatataatg
      421 atatatgggc actttcttct gtgctaggaa acggaggtag aaggggatag caagcttgag
      481 cagtaattaa aaaaaaaaaa aaagcatatt gatagatagc tcatttgtag ttaactgacg
      541 acatttatgt acataattaa aatagaagaa aattttcaaa aaaaattctc ctggtgattg
      601 ttaaggacaa gaaaacaagc catgttgtaa ccaataaatg ttacacgcct tc
//
