LOCUS       sequence-98198-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disintegrator plasmid encoding MET17 promoter driven green beetle 
            luciferase with nuclear localization signal (NLS) and ClpXP degron 
            tag (ssrA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB695
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attctattac ccccatccat actcttaatt aaccatggct ccaccaaaaa aaaaaagaaa
       61 agttgccatg ggtgggggca gcatgatgaa gagagaaaaa aatgttgttt acggccccga
      121 acccaaacac cctttgggaa actttactgc tggagaaatg ctctacaatg cccttcataa
      181 gcattcccac ataccgcaag caatattaga tgtgatgggt aatgaatcgc tgtcatatca
      241 agaatttttc gacactactg tcaagctagg acaaagtctt caaaattgtg gatacaagat
      301 gaatgatgta gtgtcgatct gtgctgagaa caataaaaga tttttcatcc ccattatttc
      361 agcttggtat attggtatgg ttgtagcacc tgttaatgaa gactacatcc cagatgaact
      421 ttgtaaagtc acgggtatat caaaaccaat actggtcttc actacaagaa aaatcttacc
      481 taaggtatta gaggtaaagg acagaactaa ttacataaag agaattataa tactagattc
      541 tgaagaaaat ctgcttggtt gcgaaagtct tcataatttt atgtcccgtt attcggataa
      601 taatcttcag acctttaagc ctctacatta cgatcctgtt gatcaagtgg cagctatttt
      661 atgttcgtca ggcacaactg gattacccaa aggtgttatg caaacgcata gaaacatttg
      721 tgttcgactt acacatgctt cggatcccag agttggaaca caacttattc ctggagtatc
      781 agttttggcg tatctgcctt tcttccatgc ttttgggttt tctataaact taggatactt
      841 tatggtgggc cttcgtgttg ttatgctaag acgatttaat caagaagtat ttttaaaagc
      901 cattcaagat tatgaagttc gaagtgtaat caacgttcca tcaacaatac tgttcttgtc
      961 gaaaagtcct ttagttgaca a
//
