LOCUS       sequence-98202-e         527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human UBE2D3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-UBE2D3i1
FEATURES             Location/Qualifiers
     source          1..527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..144)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     148..168
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
       61 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataacttcgt
      121 atagcataca ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag
      181 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttt gttcagttaa
      241 agtgcacagt ctgttctaga gacagactgt gcactttaac tgaacttttt tctcgagtac
      301 taggatccat taggcggccg cgtggataac cgtattaccg ccatgcatta gttattaata
      361 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      421 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      481 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtc
//
