LOCUS       sequence-98205-e         503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human ADAM21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-ADAM21i1
FEATURES             Location/Qualifiers
     source          1..503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..142)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     146..166
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ttgcatataa tatttcctag taactataga ggcttaatgt gcgataaaag acagataatc
       61 tgttcttttt aatactagct acattttaca tgataggctt ggatttctat aacttcgtat
      121 agcatacatt atacgaagtt ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta
      181 attgtgtgtt ttgagactat aaatatccct tggagaaaag ccttgttaac gcgcggtgac
      241 cctcgaggca gtctgtctgt cttgtcacat ttctagagaa tgtgacaaga cagacagact
      301 gcttttttgg atccattagg cggccgcgtg gataaccgta ttaccgccat gcattagtta
      361 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      421 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      481 aataatgacg tatgttccca tag
//
